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Synthesis and Characterization of Polymer for Blood Matching Test(PDF)

《南京理工大学学报》(自然科学版)[ISSN:1005-9830/CN:32-1397/N]

Issue:
2001年03期
Page:
290-294
Research Field:
Publishing date:

Info

Title:
Synthesis and Characterization of Polymer for Blood Matching Test
Author(s):
YangXujie ZhuJunwu WangXin LuLude ZhouTongbin①
School of Chemical Engineering,NUST,Nanjing 210094)
Keywords:
poly( dimethyl diallyl ammonium) chloride infrared spectrum NMR match blood
PACS:
O631
DOI:
-
Abstract:
The quaternary ammonium polyelect roly tes w ere synthesized by reaction of dimethylamine and allyl chloride, and the st ructures of monomer and polymer w ere characterized by inf rared and proton nuclear magnet ic resonance spect roscopy . T he poly( dimethyl dia-l lyl ammonium) chloride w as used to detect the incomplete IgG ant ibodies of red blood cell in human seria, and the several factors, such as concentration of sodium ethy lene diamine tet racet ic acid ( EDTA·Na2) , polyelectrolytes and red blood cell w ere discussed in the match blood test . It is found that this article presents data support ing the use of the manual poly ( dimethyl diallyl ammonium ) chloride test , a 1- minute room temperature procedures provide a rapid and more sensit ive test . It can be compared with bromeline, polybrene technique, etc. T he result s show that this method is superior to commercial methods, and equal to polybrene . T his new technic should be a valuable aid in the detect ion and identif icat ion of weak antibodies.

References:

1 马春燕. 酶法交叉配血的改进. 中国输血杂志, 1995, 8( 4) : 195~ 196
2 Fisher G A. Use of the manual po lybr ene test in the routine hospital laboratory . Transfusion,1983, 23( 2) : 152~ 154
3 忽柏林. 凝聚胺法在交叉配血试验中的应用. 中国输血杂志, 1999, 12( 2) : 87~ 89
4 Lown J A G. Polybrene technique for red cell antibody screening using micro plates. J Clin Patho l,1988, 41: 556~ 557
5 Lalezar i P. The manual polybrene test: a simple and rapid procedur e for detection of r ed cell antibodies. Transfusion, 1980, 20( 2) : 206~ 211

Memo

Memo:
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Last Update: 2013-03-25