|Table of Contents|

Fluorescence Spectra of TR Molecule Induced by Lights with Different Wavelengths


Research Field:
Publishing date:


Fluorescence Spectra of TR Molecule Induced by Lights with Different Wavelengths
YANG Tao 12 LAN Xiu-feng1 LUO Xiao-sen1 NI Xiao-wu1 XU Lin2
1. Schoolof Sciences, NUST, Nanjing 210094, China; 2. Nanjing Military Medical College, Second Military Medical University, Nanjing.210099, China
th ioredox in reductase (TR ) fluorescence spectra induced waveleng th
Mult-i funct ion grat ing spectrograph was used to measure the fluorescence spectra o f the thioredox in reductase (TR) excited by ultrav io let (UV ) light of 253. 7 nm and v ioletLED ( LightEm itting D iode) light o f 407 nm. The expermi ental resu lts indicate that the TR so lution can em it strong fluorescence induced by those two k inds of ligh.t The characterist ics of fluorescence from the TR mo lecule induced by UV light and LED light are obtained by analyzing the spectra of the TR solution and its so lven.t The results can prov ide a foundation for better understanding of the TR structures by themethod of fluorescence spectroscopy.


[ 1] Laurent T C, M oore E C, Re ichard P. Enzym atic synthesis of deoxy ribonucleotides and characterization of thioredox in, the hydrogen donor from echer ich ia co li [ J] . B J B io l Chem, 1964, 239: 3 436- 3 444.
[ 2 ] Khan I A, Luduna R F. Possible regu lation of the in vitro assem bly o f bov ine tubulin by the bov ineth ioredox in system [ J]. B iochem B iophys Acta, 1991, 1 076 ( 2): 289- 297.
[ 3] Gasdas J R, Gasdaska P Y, Gallegos A, et a.l H um an th ioerdox in reductase gene loca lization to chrom osom al po sition 12q23-q24. 1 and mRNA distribution in hum an tissue [ J]. Genom ics, 1996, 37 ( 2): 257- 259.
[ 4] Bergg renM, Ga llegosA, Gasdaska J R, et a.l Thiorefoxin and th io redox in reduc tase gene expression in hum an tum ors and ce ll lines, and the effec ts of serum stim ulation and hypox ia [ J] . Anticance r Res, 1996, 16 ( 6B ): 3 459- 3 466.
[ 5] G ladyshev V N, Stadtm an T C, H atfie ld D I, e t a l. Lev es of m a jo r selenopro teins in T cells decrease during H IV infection and low m o lecular m ass selen ium compounds increase [ J]. Proc N atl A cad Sci USA 1999, 96: 835- 839.
[ 6] Ko jima s, M a tsuk iO, Nom ura T, et a .l E levation of antioxdan t potency in the brain o fm ice by low-dose gamm aray irradiation and its e ffect on lm ethy l 1-4-pheny-l 1, 2, 3, 6- tetrahydropyr idine ( MPTP) - induced bra in dam ag e [ J]. FreeRad ic Bo ilM ed, 1999, 26 ( 3-4) : 388- 395.
[ 7] A rscoo t. L D, G rom er S, Schirm er R H, et a.l The m echanism s o f lipoam ide dehydrogenase and g lutath ione reductase and g lutath ione reductase and is d istinct from the m echan ism o f th ioredox in reductase from Escherich ia co li [ J] . ProcNa tlAcad Sc iUSA . 1997, 94: 3 621- 3 626.
[ 8] 徐江英, 许琳, 秦艳, 等. 猪脑硫氧还蛋白还原酶基因片段的序列分析[ J]. 南京军医学院学报, 2000, 22 ( 3): 152- 155.
[ 9] 陈国珍, 黄贤智, 许金钩, 等. 荧光分析法[M ]. 科学出版社, 1990.
[ 10] 孙毓庆. 分析化学[M ] . 人民卫生出版社, 1992.
[ 11] 鄢远, 刘涛, 黄坚锋. 溶液中溶菌酶的荧光光谱研究 [ J] . 化学学报, 1997, 55( 12) : 1 214- 1 218.
[ 12] 龙中儿, 钟青萍, 刘活林, 等. 卵黄免疫球蛋白的稳定性研究[ J] . 中国生物制品学杂志, 1997, 10( 4): 218 - 221.
[ 13 ] Ludw ig B, M ichae l L J. Fluorescence Spectroscopy (M e thods in Enzym o logy) [M ]. New York: Academic Press, 1997.
[ 14] 赵南明, 周海梦. 生物物理学[M ] . 北京: 高等教育出版社, 2000.


Last Update: 2013-03-03