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Enrichment Cultivation and Isolation of Biohydrogen Production Anaerobes in Fermentative Process


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Enrichment Cultivation and Isolation of Biohydrogen Production Anaerobes in Fermentative Process
LI Yong-feng~1REN Nan-qi~1YANG Chuan-ping~2HU Li-jie~1WU Yi-ning~1ZHANG Wen-qi~1
1.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;2.School of Forestry,University of Northeast Forestry,Harbin 150024,China
fermen tation hydrogen product ion H2-producing bacteria enrichment cultivat ion -i so lation methods
X 713
The enr ichmen,t iso lation and cu ltivation ofH2-produc ing bacteria (HPB ) w ith enhancement o f hydrogen product ion capacity improve hydrogen production capac ity in the system o f b iohydrogen production w ith d igesting high strength organ icw astew ater. Them ixed fermen tative system w ith act ivated sludge and design ing med ium are adopted to enrich and iso la teHPB. The b ioreactor of the con tinuous fermen tative hydrogen production is operated into the stage o f ethanol type with h igh hydrogen production y ield. The main operat ion param eters are as follow s: 4. 0~ 4. 2 pH value, 35~ 38℃ , - 100mV of oxygen reduction poten tial ( ORP) . The activated sludge w ith the dom-i nantm icrobial community ofHPB is enriched preparatorily after 40~ 50 days o f operation. The carbon source w ith glucose, fructose and maltose and the n itrogen sourcew ith peptone and beef extract ion are adopted as the design ing specialmed ium (HPB-LR ). HPB is enriched effective ly byHPBLR under the strict anaerobic cond ition. The better anaerobic techniques are to use the improved Hungater. method, inclined p lane method and cultura l bottle p late method. Abou t 94 stra ins o f HPB are iso lated and cultivated. Three stra ins ofHPB w ith h igh hydrogen production y ield and rate are obtained after select ion and characterization. These bacteria offer the importantm icrob ial germs for the fermenta tive hydrogen production.


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Last Update: 2006-06-30