|Table of Contents|

Effects of Temperature and pH on Expression of Virulence Factors from Aeromonas Hydrophila

《南京理工大学学报》(自然科学版)[ISSN:1005-9830/CN:32-1397/N]

Issue:
2006年03期
Page:
375-380
Research Field:
Publishing date:
2006-06-30

Info

Title:
Effects of Temperature and pH on Expression of Virulence Factors from Aeromonas Hydrophila
Author(s):
HU Jing~1LI Ai-hua~2HU Cheng-yu~1ZHU Da-ling~2CAI Tao-zhen~2WANG Jian-guo~2SI Ya-dong~2
1.College of Life Science,Nanchang University,Nanchang 330047,China;2.Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China
Keywords:
Aeromonas hydrophila v irulence gene temperature pH expression
PACS:
S 941, Q 78
DOI:
-
Abstract:
The effects o f temperature and pH on the expression of four viru lence factors: hemo lysin (AHH ) , aero lysin ( A erA ) , outer membrane proteins ( OMP) and adhesion ( Aha) , from A eromonas hydroph ila and on its pathogen icity are stud ied. The results show that the expression of these v iru lence factors from A. hydrophila is affected by temperature and pH in themedia. A ll four v irulence factors are h ighly expressed in A. hydrophila w hen be ing g rown at 15 e , 25 e and 37 e determ ined by RT-PCR. A t 4 e , both AHH and AerA are still expressed, but neitherOMP no rAha w as. A ll four v irulence facto rs tested here are expressed in bacteria l ce lls when being g rown at pH 5. 0 and pH 7. 0. On ly AHH expression is detected at pH 9. 0. These resu lts are supported by the resu lts from the challenge experimentsw ith crucian carp. In add ition, the study show s that the pathogen icity of the bacter ia is affected by the body temperature of fish.

References:

[ 1] Chopra A K, H ouston C W. Entero tox ins in A eromonas- assoc iated gastroen teritis [ J ]. M icrobes Infect, 1999, 1( 13): 1 129– 1 137.
[ 2] Dorm an C J. DNA superco iling and environm ental regulation of gene expression in pathog enic bacteria [ J]. Infect Immun, 1991, 59( 3): 745- 749.
[ 3] M eka lano s J J. Env ironm enta l s igna ls contro lling express ion o f v irulence determ inants in bac teria [ J]. J Bacte rio ,l 1992, 174( 1): 1- 7.
[ 4] 徐伯亥, 殷战, 吴玉深, 等. 淡水养殖鱼类暴发性传 染病致病细菌的研究[ J]. 水生生物学, 1993, 17 ( 3): 259- 265.
[ 5] W ang G , C lark C G, L iu C, et a.l Detection and charac terization of the hem o ly sin genes in Aerom onas hydrop hila and Aerom onas sobria by mu ltiplex PCR [ J]. J C linM ic robio,l 2003, 41( 8): 1 048- 1 054.
[ 6] Gonza lez-Serrano C J, Santos J A, Garcia-LopezM L, e t a.l V irulence ma rkers in A erom onas hyd rophila and Aerom onas veronii biovar sobria isolates from freshwa ter fish and from a diarrhea case [ J]. J App l M icrobio,l 2002, 93( 3): 414- 419.
[ 7] M cM ahonM A. The expression o f pro te inases and haemo lysins byA erom onas hydrophila under mod ified a-t mo spheres [ J] . J App lM icrobio,l 2000, 89( 3): 415 - 422.
[ 8] Tsa i G J, Tsai F C, Kong Z L. E ffects o f tem perature, medium composition, pH, sa lt and d isso lved oxygen on haem olysin and cytotox in production by Aeromonas hydrophila isolated from oy ste r [ J]. Int J FoodM icrobio,l 1997, 38( 2 /3): 111- 116.
[ 9] Gonzalez-RodriguezM N, Santos J A, OteroA, et a.l H em o lytic and proteo ly tic activ ities of Aerom onas hydrophila and Aeromonas veronii biova r sobria in broth and salm on ex tract a t different tem pera tures [ J]. J Food Pro t, 2004, 67( 2): 278- 284.
[ 10] M er ino S, Camprubi S, Tomas JM. E ffect of g row th tem pe rature on outerm embrane componen ts and v irulence of Aeromonas hydroph ila stra ins o f sero type B34 [ J]. Infect Immun, 1992, 60( 10): 4 343- 4 349.

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Last Update: 2006-06-30