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Analysis of Microbial Community of Activated Sludge in Different Aeration Basins within Petrochemical Industry Wastewater Treatment System by PCR-DGGE


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Analysis of Microbial Community of Activated Sludge in Different Aeration Basins within Petrochemical Industry Wastewater Treatment System by PCR-DGGE
SU Jun-feng12MA Fang1ZHAO Li-jun1WEI Li1LI Wei-guo1HOU Ning1
1.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;2.School of Environmental and Municipal Engineering,Xi’an University ofArchitecture and Technology,Xi’an 710055,China
petrochemical industry wastewater bacterial community structure polymerase chain reaction and denaturing gradient gel electrophoresis
To enhance the efficiency of A/O process for petrochemical wastewater treatment,the relationship between bacterial community structure and the pollutants loading/degrading rates are analyzed by the polymerase chain reaction and denaturing gradient gel electrophoresis(PCR-DGGE) technique.The results show: in the condition of COD loading between 424.92 mg/L and 559.10 mg/L and NH3-N between 63.60 mg/L and 100.87 mg/L,the removal rate of COD and NH3-N is low as 69.42% and 31.2% respectively.The removal rate of COD and NH3-N fluctuats with bacterial categories in the bioreactor.The profile of bacterial community in different units by PCR-DGGE show that the number of bacterium bands chang a lot and the highest similar coefficient is 36.11% and the lowest is 6.25%,and that the similarity of bacterial community structure is extremely low.The experimental results indicate that the relationship between structure of bacterial community and its functions may be elucidated with the analysis of such variation.


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Last Update: 2012-12-05