|Table of Contents|

Optimization of Fermentation Process of CoQ10 Produced by Candida Tropicalis(Strain C-1)


Research Field:
Publishing date:


Optimization of Fermentation Process of CoQ10 Produced by Candida Tropicalis(Strain C-1)
SHI Ruo-fu1XIONG Lu-lu2
1.School of Chemical Engineering,NUST,Nanjing 210094,China;2.Department of Education Evaluation,Yancheng Institute of Technology,Yancheng 224003,China
CoQ10 candida tropicalis fermentation conditions
The fermentation process of candida tropicalis(strain C-1) is optimized.The effect of some ferment conditions,including carbonaceous,nitrogenaceous,inoculating volume,pH and medium volume,on the biomass and content of CoQ10 are researched.The orthogonal test is used to analyze these fermentation conditions.The results show that nitrogenaceous is very important to CoQ10 synthesis and that pH,medium volume,and carbonaceous are in the next place.An elementary ferment condition is gained,that is,carbonaceous(glucose 26 g/L+sucrose 26 g/L),nitrogenaceous(yeast extract 7.5 g/L+peptone 7.5 g/L),pH(6.5),medium volume(40 mL),ferment time(30 h),culture temperature(30 ℃),and vitamin B1,nicotinic acid and iron are added to the medium for speeding growth.10.8 mg/L CoQ10 is obtained.


[1] 肖新才, 冯翔. CoQ10抗氧化作用研究进展[ J]. 国 外医学卫生学分册, 2003, 30( 4): 216- 221.
[2] Co ld iron A D, Sanders R A, W atkins J B, et a.l E ffects o f com bined quercetin and coenzym e Q ( 10) treatm ent on ox idative stress in norma l and diabetic rats [ J]. Chem Mo l Tox ico ,l 2002, 16 ( 4): 197- 202.
[3] 法兰克#莫瑞. 100种健康营养素完全指南[M ]. 汕 头: 汕头大学出版社. 2003. 91- 96.
[4] 邱卫华, 刘萍, 钟桂芳, 等. 粟酒裂殖酵母中CoQ10 的提取和测定方法[ J]. 仪器与发酵工业, 2004 ( 11): 31- 35.
[5] 王根华, 钱和, 肖刚. 发酵菌体中辅酶Q 的提取及 其测定方法[ J]. 无锡轻工大学学报, 2003, 22( 2): 59- 62.
[6] Soha l R S, Kam za lov S, Sum ien N, e t a.l E ffect o f coenzym e Q10 intake on endog enous coenzyme Q conten t, m itochondr ial electron transpo rt cha in, antiox idative defenses, and life span o f m ice [ J] . Free Rad ic B io lM ed, 2006, 40 ( 3): 480- 487.
[7] Anderson R F, H ille R, Sh inde S S, et a.l E lectron transfer w ithin comp lex ? . Succ inate: ubiquinone oxido reductase o f Escher ichia co li [ J] . J B io l Chem, 2005, 280 ( 39): 33 331- 33 337.


Last Update: 2012-12-19