|Table of Contents|

Experimental Research on Effect of Grape Seed Extract on Apoptosis of Prostate Cancer PC-3 Cells

《南京理工大学学报》(自然科学版)[ISSN:1005-9830/CN:32-1397/N]

Issue:
2008年06期
Page:
782-787
Research Field:
Publishing date:

Info

Title:
Experimental Research on Effect of Grape Seed Extract on Apoptosis of Prostate Cancer PC-3 Cells
Author(s):
ZHANG Li1SHANG Xue-jun2TENG Wen-hui1
1.School of Chemical Engineering,NUST,Nanjing 210094,China;2.Department of Central Laboratory,Nanjing General Hospital of Nanjing Military Region,Nanjing 210002,China
Keywords:
grape seed extract prostate cancer PC-3 cells apoptosis caspase pathway
PACS:
R285.5;R737.25
DOI:
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Abstract:
This paper studies the effect of grape seed extract(GSE) on the proliferation and apoptosis of PC-3 cells.To analyze the pathway in which GSE induces apoptosis of PC-3 cells,MTT assay is applied to test the inhibitory effect of GSE on the proliferation of PC-3 cells.Inverted microscopy and transmission electron microscopy(TEM) are used to observe the morphologic changes in PC-3 cells.The effects of GSE on the apoptosis of PC-3 cells are studied by PI and AO/EB staining,and the pathway of apoptosis is investigated by Western blot.The results show that the proliferation of PC-3 cells is inhibited in a dose-and time-dependent manner.Changes in PC-3 cells morphology are observed by inverted microscopy and TEM.PI staining assay indicats that GSE arrests PC-3 cells in the G0/G1 phase.Western blot shows that GSE induces apoptosis in the caspase-3 pathway.GSE has cytotoxicity on PC-3 cells and induces the apoptosis and necrosis of androgen-independent prostate cancer PC-3 cells in a mitochondrion-dependent manner.

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Last Update: 2008-12-30