|Table of Contents|

Experimental Study on Anaerobic Ammonia Oxidation Microorganisms and Integration of Methanogenesis with Denitrification


Research Field:
Publishing date:


Experimental Study on Anaerobic Ammonia Oxidation Microorganisms and Integration of Methanogenesis with Denitrification
CAO Jia-shun12ZHOU Wen-li3ZHANG Yu-tao1WEI Qi-xin1
1.Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes,Ministry ofEducation,Hohai University,Nanjing 210098,China;2.National Engineering Research Center of WaterResources Efficient Utilization and Engineering Safety,Nanjing 21
anaerobic ammonia oxidation denitrification sludge methanogenesis gas-lift reactors
According to the characteristic of anaerobic ammonia oxidation(ANAMMOX) bacteria,denitrification bacteria and methanogenesis bacteria,a gas-lift reactor is used to cultivate ANAMMOX sludge with inoculating denitrifying granular sludge.ANAMMOX microorganisms and integration of methanogenesis with simultaneous denitrification are researched and the disposal effect of high ammonia concentration organic wastewater is reviewed.After 106 days’ operation,the NH3-N removal efficiencies,the TN removal efficiencies,the NO-3-N removal efficiencies,the COD removal efficiencies are 45%,69%,94% and 81% respectively.In the process of reaction,the phenomenon of dephosphorization and phosphine are found and the sludge changes from the gray and black to the brown,yellow and granular.The test on PCR proves that it has better characteristic of ANAMMOX microorganisms.


[ 1] StrousM, HeijnenJ J, Kuenen JG, et a.l The se-quencing batchreactor as apowerful tool for the study of slowly growing anaerobic ammonium-oxidizing m-i cro-organisms[ J]. MicrobiologyBiotechnology, 1996, 50( 5): 589-596.
[ 2] SchmidM, TwachtmannU, KleinM, eta.l Molecular evidence for genus leveldiversityofbacteria capableof catalyzing anaerobic ammoniumoxidation[ J]. System AppliedMicrobiology, 2000, 23(1): 93-106.
[ 3] VandeGraafAA, De BruijnP, RobertsonLA, et a.l Autotrophic growth of anaerobic ammonium-oxid-i zing micro-organisms in a fluidized bed reactor[ J]. Microbiology, 1996, 142( 8): 2187-2196.
[ 4] MulderA, VandeGraafAA, RobertsonLA, et a.l Anaerobic ammoniumoxidationdiscoveredinadenitr-i fying fluidizedbedreactor[ J]. FEMSMicrobiologyE-cology, 1995, 16( 3): 177- 183.
[ 5] 金仁村, 郑平,胡宝兰, 等. 污泥颗粒化快速启动厌氧氨氧化反应器的探讨[ J]. 环境污泥与防治, 2006, 28( 10): 772- 775.
[ 6] Zhang D J. The integration of methanogenesis with denitrification and anaerobic ammoniumoxidation in anexpandedgranular sludge bed reactor[ J]. Env-i ronmentalScience, 2003, 15( 3): 423- 432.
[ 7] Wang Jian-long, Kang Jing. The characteristicsof an-aerobic ammoniumoxidation( ANAMMOX) by granu-lar sludge fromanEGSB reactor[ J]. Process Bio-chemistry, 2005, 40: 1973- 1978.
[ 8] Kluber HD, ConradR. Inhibitory effects of nitrate, nitrite, NO andN 2 OonmethanogenesisbyMethano-sarcina barkeri andMethanobacterium bryantii[ J]. FEMSMicrobiology Ecology, 1998, 25: 331-339.
[ 9] 国家环境保护总局. 水与废水监测分析方法[M]. 第4版. 北京:中国环境科学出版社, 2003.
[ 10] 祖波, 张代钧, 张萍, 等. 影响厌氧氨氧化与甲烷化反硝化耦合的因素[ J]. 应用与环境生物学报, 2007, 13(3): 438- 442.
[ 11] Akunna JC, BizeauC, MolettaR. Nitrateandnitrite reductionswithanaerobic sludge using glucose at va-riouscarbonsources: glucose, glycero,l acetic acid, lactic acid and methanol [ J]. Water Research, 1993, 27: 1303-1312.
[ 12] 郭夏丽. 厌氧生物除磷技术的基础研究[ D]. 杭州:浙江大学环境与资源学院, 2005.
[ 13] LinYF, ChenKC. Denitrificationandmethanogen-esis in a coimmobilizedmixed culture system[ J]. Water Research, 1995, 29( 1): 35-43.
[ 14] HendriksenHV, Ahring BK. Integrated removal of nitrate and carbon in an upflow anaerobic sludge blanket(UASB) reactor: operating performance[ J]. Water Research, 1996, 30( 6): 1451- 1458.
[ 15] JettenMSM, HornSJ, vanLoosdrechtMCM. To-wardsamore sustainablemunicipalwastewater trea-t ment system[ J]. Water ScienceTechnology, 1997, 35: 171- 180.
[ 16] SponzaDT, AtalayH. Simultaneousphosphorus, n-i trogenanddenitrificationremovals inbatchanaerobic / anoxic / aerobic sequence[ J]. ProcessBiochemis-try, 2005, 40: 25-34.


Last Update: 2012-11-19