|Table of Contents|

Preparation of KGM/Glutaraldehyde and Its Urease Immobilization Properties

《南京理工大学学报》(自然科学版)[ISSN:1005-9830/CN:32-1397/N]

Issue:
2009年05期
Page:
687-691
Research Field:
Publishing date:

Info

Title:
Preparation of KGM/Glutaraldehyde and Its Urease Immobilization Properties
Author(s):
ZHANG LiZHU YanWANG Lian-jun
School of Chemical Engineering,NUST,Nanjing 210094,China
Keywords:
konjac glucomannan glutaraldehyde urease immobilization spongiform carriers
PACS:
Q814.2
DOI:
-
Abstract:
The spongiform konjac glucomannan(KGM) carrier is prepared with KGM and glutaraldehyde,and urease is immobilized on the carrier.The optimum condition for the immobilization of urease is measured and the characterizations of immobilized urease are compared with the free urease.The results show that the optimum condition for KGM carrier preparation is to dissolve 1.5 g refined konjac flour into 30 mL 0.1 mol/L NaOH,freeze the obtained substance and treat it insolubly using 50% 0.1mol/L NaOH in ethanol,and activate the carrier with 5% glutaraldehyde for 1.5 h.Reacting the carrier with urease for 1h at 4℃ can achieve the highest enzyme activity.For the immobilized urease,the optimum temperature is 60℃,the optimum pH is 7.5,and the Michaelis constant(Km) is 0.001 87 mol/L.The results also show that the stability of the carrier can be greatly improved through freezing treatment.

References:

[ 1] 吴晓霞, 李建科,余朝舟. 魔芋葡甘聚糖-壳聚糖-羧甲基纤维素钠复合可食性保鲜膜研究[ J]. 食品工业科技, 2008(2): 236- 238.
[ 2] 张峰, 陈庆华, 潘兴华, 等. 羟基磷灰石/魔芋葡甘聚糖复合材料的制备和表征[ J]. 中国组织工程研究与临床康复, 2008, 12( 6): 1072-1076.
[ 3] 许东颖, 盛家荣, 李光吉. 魔芋葡甘聚糖的改性及其在生物材料领域的应用概况[ J]. 材料导报, 2008, 22( 11): 47-50.
[ 4] 庞杰,林琼,张甫生, 等. 魔芋葡甘聚糖功能材料研究与应用进展[ J]. 结构化学, 2003, 22(6): 633-642.
[ 5] 邹群,朱丹丹,夏服宝. 交联魔芋葡甘聚糖水凝胶包裹尿素的缓释效果[ J]. 江苏化工, 2005, 33(2): 41-43.
[ 6] 陈家任,贾成禹. 魔芋多糖固定化酿酒酵母生理生化特性的研究[ J]. 微生物学报, 1991, 31( 5): 390-395.
[ 7] PerolsC, PiffautB, Scher J. The potential of enzyme entrapment inkonjac cold-melting gel beads[ J]. En-zyme andMicrobialTechnology, 1997, 20: 57- 60.
[ 8] 陈家任,贾成禹. 一种魔芋多糖固定化微生物细胞和酶的新方法[ P]. 中国, 1053089A. 1991-07- 17.
[ 9] 贾成禹, 陈家任, 古明选. 魔芋葡甘露聚糖新载体制备和对葡萄糖淀粉酶的固定化[ J]. 生物化学杂志, 1991, 7( 3): 359-364.
[ 10] 岳蔚然, 唐学友. 卡拉胶-魔芋多糖复配胶固定化微生物载体及其应用[ P]. 中国, 1234444. 1999-05- 17.
[ 11] 罗侃, 崔有宏,王绪民. 脲酶Km值的比色测定法及其评价[ J]. 兰后卫生, 1995, 16(1): 1-4.
[ 12] 王梅. 大豆产品脲酶活力定性测定研究[ J]. 安徽农业科学, 1999, 27( 1): 89-90.
[ 13] 扶惠华,李小方,田庭亮. 奈氏试剂测定脲酶活性方法的改进[ J]. 植物生理通讯, 1996, 32( 6): 35-39.

Memo

Memo:
-
Last Update: 2012-11-19