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Degrading Characteristics of Two Petrolecm-degrading Strains


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Degrading Characteristics of Two Petrolecm-degrading Strains
ZHANG Lu-jinYANG QianCHEN Zhong-xiangZHANG Xiang-dong
Department of Life Science and Technology,Harbin Institute of Technology,Harbin 150001,China
petroleum biodegradation degrading characteristics orthogonal tests gas chromatography-mass spectrum
To study the bioremediation technology of the petroleum contaminated soil,the influential factors of petroleum biodegradation and the optimizing degradation conditions of two petrolecm-degrading strains of Pseudomonas sp.ZL13 and Alcaligenes sp.ZL21 are researched by orthogonal test.Their degradation ability for different components of the crude oil is studied by the technology of gas chromatography-mass spectrum(GC-MS).The results show that the influential factors of two strains are oil mass concentration and temperature.Under the optimum conditions,the crude oil degradation rates by ZL13 and ZL21 after seven days’ incubation is 72.68% and 73.10%.The two strains can effectively degrade most components of the crude oil.The degradation ability of strain ZL21 is better than that of strain ZL13.


[ 1] 袁红莉, 杨金水, 王占生, 等. 降解石油微生物菌种的筛选及降解特性[ J]. 中国环境科学, 2003,23( 2): 157- 161.
[ 2] A ltas RM. Petro leum b iodeg radation and o il spill b ioremediation[A]. Sym posium on B ioremediation and B ioprocessing,D iv ision of Petro leum Chem istry, Inc. 205thNationa lMeeting o f Amer ican Chem ica l Society [ C ].Denver, USA: Denver, CO, 1993: 236- 237.
[ 3] 金志刚, 张彤, 朱怀兰. 污染物生物降解[M ]. 上海: 华东理工大学出版社, 1997.
[ 4] 李习武, 刘志培. 石油烃类的微生物降解[ J]. 微生物学报, 2002, 6( 42) : 764- 767.
[ 5] Adew a le J, Lam bo T, Pate l R. Iso la tion and characterization o f a b ipheny l utilizing psychro troph ic bacter-ium, hydrogenophaga taen iosp ira lis IA3-A, that com etabolize d ichlorob ipheny ls and po lych lo rinated b ipheny lcong eners in aroc lor [ J]. J Basic M icrob io,l 2006,46( 2): 94- 107.
[ 6] Cristiana C L, V incent G, Dan ie lle R, et a.l Anaerobicn-alkane m etabo lism by a su lfate- reduc ing bacter ium,desu lfa tibacillum a liphatic ivorans stra in CV2803T [ J ].Applied and Env ironm entalM icrob iology, 2005, 71( 7):3458- 3467.
[ 7] M oham edM E, A-lDousaryM, H am zah R Y, et a.l Isola tion and character ization o f ind igenous thermophilicbacter ia active in na tura l a ttenuation of bio-hazardouspetrochem ica l pollutants[ J] . In ternational B iodeter ioration& B iodegradation, 2006, 58: 213- 223.
[ 8] 许雄飞, 丁庆云, 张秀影, 等. 快速溶剂萃取红外分光光度法测定土壤中石油类[ J]. 中国环境监测,2008, 24( 3): 4- 6.
[ 9] 杨雪莲, 李凤梅, 刘婉婷, 等. 高效石油降解菌的筛选及其降解特性[ J]. 农业环境科学学报, 2008,27( 1): 230- 233.
[ 10] Huang Q Z, Wang SM, Huang J F, e t a.l Study on theheterogeneous degradation of chitosan w ith hydrogen peroxide under the cata lysis o f phosphotungstic ac id [ J].Carbohydrate Po lymers, 2007, 68( 4): 761- 765.
[ 11] Rouven E, Andrea V, Ange lo R, et a.l Im proved assessment of b iodegrada tion extent and prediction o f petroleumqua lity [ J]. O rganic Geochem istry, 2007,38( 12): 2111- 2130.
[ 12] G reen S B, Salkind N J, Akey T M. U sing SPSS fo rW indow s: Analyzing and understanding data [M ].New Jersey, USA: PrinnticeH al,l 2000.
[ 13] Te llez G T, N irm a lakhandan N, Gardea-To rresdey JL. Comparison of purge and trap GC /M S and spectrophotometry for m onitor ing petro leum hydrocarbon degradation in o ilfield produced waters[ J]. M icrochem -ica l Journa,l 2005, 81( 1): 12- 18.
[ 14] ZhangH F, Su X L, Sun D K, et a .l Investigation ondeg radation of po lyethy lene to o il in a con tinuous supercriticalwa ter reactor[ J]. Journal of Fue l Chem istryand Technology, 2007, 35( 4): 487- 491.
[ 15] Ju?rezM J B, Zafra-G?me z A, Luz?n-Toro B, et a.lGas chrom atographic-m ass spectrom etr ic study of thedegradation of pheno lic compounds in wastewa ter o liv eo il by azotobacter chroo coccum [ J]. B ioresource Technology, 2008, 99( 7): 2392- 2398.
[ 16] Saadoun I. Iso lation and character ization of bacte riafrom c rude pe tro leum o il contam ina ted so il and the irpotentia l to deg rade d iese l fue l[ J] . Journa l o f BasicM icrob io logy, 2002, 42( 6): 420- 428.
[ 17] A tlas R M. M icrob ial deg radation of pe tro leum hydrocarbons: An env ironmenta l perspective [ J]. M icrob ia lRev, 1981, 45( 1): 180- 199.
[ 18] 贾燕, 尹华, 彭辉, 等. 石油降解菌株的筛选、初步鉴定及其特性[ J]. 暨南大学学报( 自然科学版),2007, 28( 3): 296- 301.
[ 19] 周海霞, 单爱琴, 王莉淋, 等. 石油降解菌的筛选及其降解效率的研究[ J] . 环境科学与技术, 2008,31( 10) : 56- 58


Last Update: 2012-11-02