[1]杨 涛,*,兰秀风,等.两种激发光诱导的硫氧还蛋白还原酶分子荧光光谱研究[J].南京理工大学学报(自然科学版),2005,(01):55-58.
 YANG Tao,LAN Xiu-feng,LUO Xiao-sen,et al.Fluorescence Spectra of TR Molecule Induced by Lights with Different Wavelengths[J].Journal of Nanjing University of Science and Technology,2005,(01):55-58.
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两种激发光诱导的硫氧还蛋白还原酶分子荧光光谱研究
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《南京理工大学学报》(自然科学版)[ISSN:1005-9830/CN:32-1397/N]

卷:
期数:
2005年01期
页码:
55-58
栏目:
出版日期:
2005-02-28

文章信息/Info

Title:
Fluorescence Spectra of TR Molecule Induced by Lights with Different Wavelengths
作者:
杨 涛1 2* 兰秀风1 骆晓森1 倪晓武1 许 琳2
1. 南京理工大学理学院, 江苏南京210094;
2. 第二军医大学南京军医学院, 江苏南京210099
Author(s):
YANG Tao 12 LAN Xiu-feng1 LUO Xiao-sen1 NI Xiao-wu1 XU Lin2
1. Schoolof Sciences, NUST, Nanjing 210094, China; 2. Nanjing Military Medical College, Second Military Medical University, Nanjing.210099, China
关键词:
硫氧还蛋白还原酶 荧光光谱 激发波长
Keywords:
th ioredox in reductase (TR ) fluorescence spectra induced waveleng th
分类号:
Q55
摘要:
分别以波长为 253. 7nm的紫外光及波长为 407nm的紫色发光二极管为激发光源, 诱导硫氧还蛋白还原酶(thioredoxinreductase,TR)分子产生荧光,用组合式多功能光栅光谱仪记录荧光谱。通过分析比较TR溶液与溶剂的荧光光谱,得到了这 2种激发光诱导的TR分子荧光光谱的特性。该文的研究结果为进一步将荧光光谱法用于TR的定性、定量分析乃至结构分析提供了依据。
Abstract:
Mult-i funct ion grat ing spectrograph was used to measure the fluorescence spectra o f the thioredox in reductase (TR) excited by ultrav io let (UV ) light of 253. 7 nm and v ioletLED ( LightEm itting D iode) light o f 407 nm. The expermi ental resu lts indicate that the TR so lution can em it strong fluorescence induced by those two k inds of ligh.t The characterist ics of fluorescence from the TR mo lecule induced by UV light and LED light are obtained by analyzing the spectra of the TR solution and its so lven.t The results can prov ide a foundation for better understanding of the TR structures by themethod of fluorescence spectroscopy.

参考文献/References:

[ 1] Laurent T C, M oore E C, Re ichard P. Enzym atic synthesis of deoxy ribonucleotides and characterization of thioredox in, the hydrogen donor from echer ich ia co li [ J] . B J B io l Chem, 1964, 239: 3 436- 3 444.
[ 2 ] Khan I A, Luduna R F. Possible regu lation of the in vitro assem bly o f bov ine tubulin by the bov ineth ioredox in system [ J]. B iochem B iophys Acta, 1991, 1 076 ( 2): 289- 297.
[ 3] Gasdas J R, Gasdaska P Y, Gallegos A, et a.l H um an th ioerdox in reductase gene loca lization to chrom osom al po sition 12q23-q24. 1 and mRNA distribution in hum an tissue [ J]. Genom ics, 1996, 37 ( 2): 257- 259.
[ 4] Bergg renM, Ga llegosA, Gasdaska J R, et a.l Thiorefoxin and th io redox in reduc tase gene expression in hum an tum ors and ce ll lines, and the effec ts of serum stim ulation and hypox ia [ J] . Anticance r Res, 1996, 16 ( 6B ): 3 459- 3 466.
[ 5] G ladyshev V N, Stadtm an T C, H atfie ld D I, e t a l. Lev es of m a jo r selenopro teins in T cells decrease during H IV infection and low m o lecular m ass selen ium compounds increase [ J]. Proc N atl A cad Sci USA 1999, 96: 835- 839.
[ 6] Ko jima s, M a tsuk iO, Nom ura T, et a .l E levation of antioxdan t potency in the brain o fm ice by low-dose gamm aray irradiation and its e ffect on lm ethy l 1-4-pheny-l 1, 2, 3, 6- tetrahydropyr idine ( MPTP) - induced bra in dam ag e [ J]. FreeRad ic Bo ilM ed, 1999, 26 ( 3-4) : 388- 395.
[ 7] A rscoo t. L D, G rom er S, Schirm er R H, et a.l The m echanism s o f lipoam ide dehydrogenase and g lutath ione reductase and g lutath ione reductase and is d istinct from the m echan ism o f th ioredox in reductase from Escherich ia co li [ J] . ProcNa tlAcad Sc iUSA . 1997, 94: 3 621- 3 626.
[ 8] 徐江英, 许琳, 秦艳, 等. 猪脑硫氧还蛋白还原酶基因片段的序列分析[ J]. 南京军医学院学报, 2000, 22 ( 3): 152- 155.
[ 9] 陈国珍, 黄贤智, 许金钩, 等. 荧光分析法[M ]. 科学出版社, 1990.
[ 10] 孙毓庆. 分析化学[M ] . 人民卫生出版社, 1992.
[ 11] 鄢远, 刘涛, 黄坚锋. 溶液中溶菌酶的荧光光谱研究 [ J] . 化学学报, 1997, 55( 12) : 1 214- 1 218.
[ 12] 龙中儿, 钟青萍, 刘活林, 等. 卵黄免疫球蛋白的稳定性研究[ J] . 中国生物制品学杂志, 1997, 10( 4): 218 - 221.
[ 13 ] Ludw ig B, M ichae l L J. Fluorescence Spectroscopy (M e thods in Enzym o logy) [M ]. New York: Academic Press, 1997.
[ 14] 赵南明, 周海梦. 生物物理学[M ] . 北京: 高等教育出版社, 2000.

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备注/Memo

备注/Memo:
作者简介: 杨涛( 1975- ) , 女, 青海西宁人, 讲师, 主要研究方向: 分子生物医学, E-m a il:shenzh@ m ail. njust. edu. cn。
更新日期/Last Update: 2013-03-03