[1]李永峰,任南琪,杨传平,等.生物制氢系统产氢菌的富集培养与分离技术[J].南京理工大学学报(自然科学版),2006,(03):366-370.
 LI Yong-feng~,REN Nan-qi~,YANG Chuan-ping~,et al.Enrichment Cultivation and Isolation of Biohydrogen Production Anaerobes in Fermentative Process[J].Journal of Nanjing University of Science and Technology,2006,(03):366-370.
点击复制

生物制氢系统产氢菌的富集培养与分离技术
分享到:

《南京理工大学学报》(自然科学版)[ISSN:1005-9830/CN:32-1397/N]

卷:
期数:
2006年03期
页码:
366-370
栏目:
出版日期:
2006-06-30

文章信息/Info

Title:
Enrichment Cultivation and Isolation of Biohydrogen Production Anaerobes in Fermentative Process
作者:
李永峰1 任南琪1 杨传平2 胡立杰1 吴忆宁1 张文启1
1. 哈尔滨工业大学市政环境工程学院, 黑龙江哈尔滨150090; 2. 东北林业大学林学院, 黑龙江哈尔滨150024
Author(s):
LI Yong-feng~1REN Nan-qi~1YANG Chuan-ping~2HU Li-jie~1WU Yi-ning~1ZHANG Wen-qi~1
1.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;2.School of Forestry,University of Northeast Forestry,Harbin 150024,China
关键词:
发酵 生物制氢 产氢菌 富集培养 分离
Keywords:
fermen tation hydrogen product ion H2-producing bacteria enrichment cultivat ion -i so lation methods
分类号:
X 713
摘要:
从生物制氢反应器中富集、分离培养发酵产氢细菌并发挥其最大产氢能力,可以提高高浓度有机废水制氢系统的产氢性能。采用活性污泥混合培养发酵反应器和设计培养基研究产氢菌的富集培养和分离。将连续流发酵法生物制氢系统的运行参数调整如下:pH值为4.0~4.2,温度为35~38℃,氧化还原电位(ORP)为-100 mV,反应器运行40~50 d,使之达到产氢最佳的乙醇型发酵阶段,初步富集以产氢菌为优势群落的活性污泥;以葡萄糖、果糖和麦牙糖为碳源,以蛋白胨和牛肉膏为氮源,设计HPB-LR培养基,严格厌氧条件下,可以有效地富集培养产氢发酵细菌。采用改良Hungater技术、厌氧管斜面法和平板培养瓶厌氧技术等3种厌氧培养技术分离培养产氢菌效果最佳。获得产氢菌94株,这些菌株经过筛选鉴定,获得3株高效产氢菌。这些菌株为发酵法生物制氢工程提供了宝贵的微生物种质资源。
Abstract:
The enr ichmen,t iso lation and cu ltivation ofH2-produc ing bacteria (HPB ) w ith enhancement o f hydrogen product ion capacity improve hydrogen production capac ity in the system o f b iohydrogen production w ith d igesting high strength organ icw astew ater. Them ixed fermen tative system w ith act ivated sludge and design ing med ium are adopted to enrich and iso la teHPB. The b ioreactor of the con tinuous fermen tative hydrogen production is operated into the stage o f ethanol type with h igh hydrogen production y ield. The main operat ion param eters are as follow s: 4. 0~ 4. 2 pH value, 35~ 38℃ , - 100mV of oxygen reduction poten tial ( ORP) . The activated sludge w ith the dom-i nantm icrobial community ofHPB is enriched preparatorily after 40~ 50 days o f operation. The carbon source w ith glucose, fructose and maltose and the n itrogen sourcew ith peptone and beef extract ion are adopted as the design ing specialmed ium (HPB-LR ). HPB is enriched effective ly byHPBLR under the strict anaerobic cond ition. The better anaerobic techniques are to use the improved Hungater. method, inclined p lane method and cultura l bottle p late method. Abou t 94 stra ins o f HPB are iso lated and cultivated. Three stra ins ofHPB w ith h igh hydrogen production y ield and rate are obtained after select ion and characterization. These bacteria offer the importantm icrob ial germs for the fermenta tive hydrogen production.

参考文献/References:

[ 1] Lev in D B P, Love L M. B iohydrogen production: Prospects and lim itations to practica l application [ J]. Int J H ydrogen Energy, 2004, 29 ( 2): 173- 185.
[ 2] LiYong feng, Ren Nanq,i Y ang Chuanping, et a.l The mo lecular character ization and hydrogen production of a new spec ies o f anaerobe [ J]. Journal of Env ironm enta l Science andH ea lth, Part A, 2005, 40 ( 10): 1 929- 1 938.
[ 3] Tanisho S, Ishiwa ta Y. Continuous hydrog en production from m olasses by the bacter ium Ente robac ter aerogenes [ J]. Int J H ydrogen Energy, 1994, 19 ( 10) : 807- 812.
[ 4] 李建政. 有机废水发酵法生物制氢技术研究[ D]. 哈尔滨: 哈尔滨工业大学市政环境工程学 院, 1999.
[ 5] Ren Nanq,i W ang Bao zhen, H uang Junl.i Ethano-l type fermentation from carbohydrate in h igh rate acidogen ic reacto r [ J]. B iotechno l B ioeng, 1997, 54 ( 5) : 428 - 433.
[ 6] Hunga terR, E. M ethods in m icrob io logy [M ]. New York: Academ ic press, 1969. 117- 132.
[ 7] 李永峰, 任南琪, 陈瑛, 等. 发酵产氢菌分离培养 的厌氧实验操作技术[ J]. 哈尔滨工业大学学报, 2004, 36( 12): 1 589- 1 592.
[ 8] 国家环境保护总局《水和废水监测分析方法》编委 会. 水和废水监测分析方法[M ] . 北京: 中国环境 科学出版社, 2002.
[ 9] 李永峰, 任南琪, 杨传平, 等. 利用16S rDNA 测序 快速鉴定废水生物处理系统目标细菌[ J] . 哈尔滨 工程大学学报, 2005, 26( 6): 806- 810.

备注/Memo

备注/Memo:
基金项目: 国家"9730"项目( G2000026402); 国家自然科学基金( 30470054) 作者简介: 李永峰( 1961- ), 男, 黑龙江哈尔滨人, 副教授, 博士, 主要研究方向: 废水处理与生物能源, E-mail: dr_ly@f 163. com; 通讯作者: 任南琪( 1959- ), 男, 江苏盐城人, 教授, 博士生导师, 长江特聘教授, 主要研究方向: 环境科学与工程, E-mail: rnq@ h it. edu. cn。
更新日期/Last Update: 2006-06-30