[1]胡 靖,李爱华,胡成钰,等.温度和pH值对嗜水气单胞菌毒力基因表达的影响[J].南京理工大学学报(自然科学版),2006,(03):375-380.
 HU Jing~,LI Ai-hua~,HU Cheng-yu~,et al.Effects of Temperature and pH on Expression of Virulence Factors from Aeromonas Hydrophila[J].Journal of Nanjing University of Science and Technology,2006,(03):375-380.
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温度和pH值对嗜水气单胞菌毒力基因表达的影响
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《南京理工大学学报》(自然科学版)[ISSN:1005-9830/CN:32-1397/N]

卷:
期数:
2006年03期
页码:
375-380
栏目:
出版日期:
2006-06-30

文章信息/Info

Title:
Effects of Temperature and pH on Expression of Virulence Factors from Aeromonas Hydrophila
作者:
胡 靖1 李爱华2 胡成钰1 朱大玲2 蔡桃珍2 汪建国2 司亚东2
1. 南昌大学生命科学院, 江西南昌330047; 2. 中国科学院水生生物研究所, 湖北武汉430072
Author(s):
HU Jing~1LI Ai-hua~2HU Cheng-yu~1ZHU Da-ling~2CAI Tao-zhen~2WANG Jian-guo~2SI Ya-dong~2
1.College of Life Science,Nanchang University,Nanchang 330047,China;2.Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China
关键词:
嗜水气单胞菌 毒力基因 温度 pH 表达
Keywords:
Aeromonas hydrophila v irulence gene temperature pH expression
分类号:
S 941, Q 78
摘要:
为了解培养温度和pH值对嗜水气单胞菌的毒力基因表达以及致病性的影响,利用反转录PCR(RT-PCR)对4种基因的表达进行了研究。研究结果显示嗜水气单胞菌毒力基因的表达受温度和pH值影响,其中溶血素(AHH)、气溶素(AerA)、外膜蛋白(OMP)和粘附素(Aha)毒力基因在15℃、25℃和37℃下均能高效表达,在4℃AHH和AerA两基因也能持续表达,但OMP和Aha两基因的表达停止。在中性或偏酸性的条件下(pH5.0和pH7.0)4种毒力基因都得以表达,在碱性条件下(pH9.0)4种基因中只有AHH得以表达,而Aha等其它3个基因的表达减弱或停止。基因表达的研究结果得到鲫鱼攻毒试验结果的支持。此外,研究还表明细菌的毒力与鱼的体温也有关系。
Abstract:
The effects o f temperature and pH on the expression of four viru lence factors: hemo lysin (AHH ) , aero lysin ( A erA ) , outer membrane proteins ( OMP) and adhesion ( Aha) , from A eromonas hydroph ila and on its pathogen icity are stud ied. The results show that the expression of these v iru lence factors from A. hydrophila is affected by temperature and pH in themedia. A ll four v irulence factors are h ighly expressed in A. hydrophila w hen be ing g rown at 15 e , 25 e and 37 e determ ined by RT-PCR. A t 4 e , both AHH and AerA are still expressed, but neitherOMP no rAha w as. A ll four v irulence facto rs tested here are expressed in bacteria l ce lls when being g rown at pH 5. 0 and pH 7. 0. On ly AHH expression is detected at pH 9. 0. These resu lts are supported by the resu lts from the challenge experimentsw ith crucian carp. In add ition, the study show s that the pathogen icity of the bacter ia is affected by the body temperature of fish.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 中国科学院水生生物研究所知识创新前沿项目( 220317) 作者简介: 胡靖( 1981- ), 女, 江西南昌人, 硕士生, 主要研究方向: 鱼类微生物学, E-mail:jean811115@ 163. com; 通讯作者: 李爱华( 1963- ), 男, 副研究员, 博士, 主要研究方向: 鱼类微生物学, E-mail:liaihua@ihb.ac. cn。
更新日期/Last Update: 2006-06-30