[1]李 清,侯丽雅,朱 丽,等.微流体数字化喷射制备细胞微胶囊及表征[J].南京理工大学学报(自然科学版),2014,38(01):89-93.
 Li Qing,Hou Liya,Zhu Li,et al.Preparation and characterization of cell microcapsules based on digital injection of micro-fluidic technology[J].Journal of Nanjing University of Science and Technology,2014,38(01):89-93.
点击复制

微流体数字化喷射制备细胞微胶囊及表征
分享到:

《南京理工大学学报》(自然科学版)[ISSN:1005-9830/CN:32-1397/N]

卷:
38卷
期数:
2014年01期
页码:
89-93
栏目:
出版日期:
2014-02-28

文章信息/Info

Title:
Preparation and characterization of cell microcapsules based on digital injection of micro-fluidic technology
作者:
李 清12侯丽雅1朱 丽1章维一1
1.南京理工大学 机械工程学院,江苏 南京 210094; 2.中北大学 机械工程与自动化学院,山西 太原 030051
Author(s):
Li Qing12Hou Liya1Zhu Li1Zhang Weiyi1
1.School of Mechanical Engineering,NUST,Nanjing 210094,China; 2.School of Mechanical Engineering and Automation,North University of China,Taiyuan 030051,China
关键词:
微流体 数字化喷射 细胞 微胶囊
Keywords:
micro-fluids digital micro-injection cells microcapsules
分类号:
Q813; R944.5
摘要:
为了探究脉冲喷射制备细胞微胶囊的粒径变化规律和控制方法,采用微流体数字化喷射技术进行了细胞微胶囊的制备研究。制备的细胞微胶囊平均粒径可小至28 μm,相应的标准偏差小于5 μm,具有较好的单分散性,通过改变驱动电压和微喷嘴内径可方便地控制细胞微胶囊的粒径大小和分布。微流体数字化喷射后细胞存活率达93%,对细胞活性影响小,适于细胞微胶囊的制备。脉冲喷射法制备细胞微胶囊,对于细胞微胶囊的后续自动化操作处理具有应用价值。
Abstract:
Based on the digital injection of micro-fluidic technology,cell microcapsules preparation is done to determine the changing law and control method of the cell microcapsules diameters.The mean diameter of the resultant microcapsules can reach less than 28 μm,the standard deviation is less than 5 μm,and the diameters are well monodisperse.The cell microcapsule diameter and the distribution can be conveniently changed by adjusting the driving voltage and the diameter of the micro nozzle.The survival of the cells in microcapsule is about 94%.The viability of the cells is slightly influenced by the cell microcapsulation.The prepation technology based on the digital injection of micro-fluide is suitable for the cell encapsulation.This supplies application value to deal with the cell microcapsulation automatically in the follow-up work.

参考文献/References:

[1] Chang T M S.Therapeutic applications of polymeric artificial cells[J].Nature Reviews Drug Discovery,2005,4:221-235.
[2]Leal-Egaña A,Fritsch A,Heidebrecht F,et al.Tuning liver stiffness against tumours:An in vitro study using entrapped cells in tumour-like microcapsules[J].Journal of the Mechanical Behavior of Biomedical Materials,2012,5(9):113-121.
[3]Yu Weiting,Song Huiyi,Ma Xiaojun,et al.Study on membrane characteristics of alginate-chitosan microcapsule with cell growth[J].Journal of Membrane Science,2011,377(7):214-220.
[4]Zhang Wujie,Zhao Shuting,Rao,Wei,et al.A novel core-shell microcapsule for encapsulation and 3D culture of embryonic stem cells[J].Journal of Materials Chemistry B,2013,2(1):1002-1009.
[5]Mendes A C,Baran E T,Lisboa P,et al.Microfluidic fabrication of self-assembled peptide-polysaccharide microcapsules as 3D environments for cell culture[J].Biomacromolecules,2012,12(13):4039-4048.
[6]Xiao Jing,Zhang Ying,Wang Jianzheng,et al.Monitoring of cell viability and proliferation in hydrogel-encapsulated system by resazurin assay[J].Applied Biochemistry and Biotechnology,2010,162(7):1996-2007.
[7]Tibbitt M W,Anseth K S.Hydrogels as extracellular matrix mimics for 3D cell culture[J].Biotechnol Bioeng,2009,103:655-663.
[8]Koster S.Drop-based microfluidic devices for encapsulation of single cells[J].Lab Chip,2008,8:1110-1115.
[9]Jean-Michel R,Xavier B,Hamza Z,et al.Progress technology in microencapsulation methods for cell therapy[J].Biotechnological Progress,2009,25(4):946-963.
[10]Zhang Weiyi,Hou Liya.Scientific and technology problems of digitalization of microfliuds(PartⅠ):Concepts,methods and results[J].Scientific and Technology Review,2005,23(8):4-9.
[11]Goosen M F A,O'Shea G M,Sun A M F.Microencapsulation of living tissue and cells[P].US4806355 A,1987-06-08.
[12]Zhang Wujie,He Xiaoming.Encapsulation of living cells in small alginate microcapsules by electrostatic spraying:A parametric study[J].Journal of Biomechanical Engineering,2009,7(131):074515-1.
[13]Alexander K,Jesse G,Ethan T,et al.High-throughput generation of hydrogel microbeads with varying elasticity for cell encapsulation[J].Biomaterials,2011,32:1477-1483.

备注/Memo

备注/Memo:
收稿日期:2012-07-27 修回日期:2012-12-31
基金项目:国家自然科学基金(51175268; 11102090); 教育部博士点专项基金(20113219110004)
作者简介:李清(1966-),男,博士生,副教授,主要研究方向:微流体系统和生物活性物质固定化技术,E-mail:liqingliqing999@163.com; 通讯作者:侯丽雅(1954-),女,教授,主要研究方向:微系统与生物制造工程,E-mail:houliya@hotmail.com。
引文格式:李清,侯丽雅,朱丽,等.微流体数字化喷射制备细胞微胶囊及表征[J].南京理工大学学报,2014,38(1):89-93.
投稿网址:http://njlgdxxb.paperonce.org
更新日期/Last Update: 2014-02-28